Comparison of cell proliferation and epigenetic modification of gene expression patterns in canine foetal fibroblasts and adipose tissue-derived mesenchymal stem cells.
Objectives: This study compared rate of cell proliferation, viability, cell size, expression patterns of genes related to pluripotency and epigenetic modification between canine foetal fibroblasts (cFF) and canine adipose tissue-derived mesenchymal stem cells (cAd-MSC). Materials and methods: Proliferation pattern, cell viability as well as cell size at each passage of cFF
and cAd-MSC were measured when cultures reached confluence. In addition, real-time PCR was performed to investigate expression of Dnmt1,
HDAC1, OCT4, SOX2, BAX, BCL2 genes with reference to b-actin gene expression as an endogenous control in both cell lines.
Results: cFF and cAd-MSC differed in number of generations, but not in doubling times, at all passages. Mean cell size of cAd-MSC was significantly
smaller than that of cFF. Cell viability was significantly lower in cFFs and apoptotic level was significantly lower in cAd-MSC compared to passage-
matched cFF. In the expression of genes related to pluripotency and epigenetic modification, level of HDAC1 in cAd-MSC was significantly
higher than in cFF, but expression of Dnmt1 did not differ between the two groups. OCT4 and SOX2 were significantly more highly expressed in
cAd-MSC compared to cFF.
Conclusions: cAd-MSC have higher stem-cell potential than cFF in terms of proliferation patterns, epigenetic modification and pluripotency, thus cAd-
MSC could be more appropriate than cFF as donors of nuclei in somatic cell nuclear transfer for transgenesis
발행연도 : 2012
저자 : Oh HJ, Park EJ, Lee SY, Soh JW, Kong IS, Choi SW, Ra JC, Kang SK, Lee BC.
출처 : Cell Prolif
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