Method of separating and culturing adult stem cells derived from human amniotic epithelial cells
This patent pertains to the method of separating improved human amnion-derived adult stem cells. To be more specific, it relates to the method of manufacturing large amounts of adult stem cells by acquiring amniotic epithelial cells, which are single cells, from human amniotic tissue through dithiothreitol (DTT) and low-concentration trypsin treatment and adding Rho-associated protein kinase (ROCK) inhibitor during the cultivation of the said amniotic epithelial cells.
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