The therapeutic efficacy of human adipose tissue-derived mesenchymal stem cells on experimental autoimmune hearing loss in mice.

요약 : 

Autoimmune inner ear disease is characterized by progressive, bilateral
although asymmetric, sensorineural hearing loss. Patients with autoimmune
inner ear disease had higher frequencies of interferon-c-producing
T cells than did control subjects tested. Human adipose-derived mesenchymal stem cells (hASCs) were recently found to suppress effector T cells and inflammatory responses and therefore have beneficial effects in various autoimmune diseases. The aim of this study was to examine the
immunosuppressive activity of hASCs on autoreactive T cells from the
experimental autoimmune hearing loss (EAHL) murine model. Female
BALB/c mice underwent b-tubulin immunization to develop EAHL; mice
with EAHL were given hASCs or PBS intraperitoneally once a week for 6
consecutive weeks. Auditory brainstem responses were examined over
time. The T helper type 1 (Th1)/Th17-mediated autoreactive responses
were examined by determining the proliferative response and cytokine
profile of splenocytes stimulated with b-tubulin. The frequency of regulatory
T (Treg) cells and their suppressive capacity on autoreactive T cells
were also determined. Systemic infusion of hASCs significantly improved
hearing function and protected hair cells in established EAHL. The hASCs
decreased the proliferation of antigen-specific Th1/Th17 cells and induced
the production of anti-inflammatory cytokine interleukin-10 in splenocytes.
They also induced the generation of antigen-specific CD4+ CD25+ Foxp3+ Treg cells with the capacity to suppress autoantigen-specific T-cell responses. The experiment demonstrated that hASCs are one of the important regulators of immune tolerance with the capacity to suppress effector T cells and to induce the generation of antigen-specific
Treg cells.

발행연도 : 2011. May

저자 : Zhou Y, Yuan J, Zhou B, Lee AJ, Lee AJ, Ghawji M Jr, Yoo TJ

출처 : Immunology

소스 : 지방 MSC

Leave a reply