Method of separating and culturing adult stem cells derived from human amniotic epithelial cells

This patent pertains to the method of separating improved human amnion-derived adult stem cells. To be more specific, it relates to the method of manufacturing large amounts of adult stem cells by acquiring amniotic epithelial cells, which are single cells, from human amniotic tissue through dithiothreitol (DTT) and low-concentration trypsin treatment and adding Rho-associated protein kinase (ROCK) inhibitor during the cultivation of the said amniotic epithelial cells.

The stem cells derived from human amniotic epithelial cells according to this patent provide an efficient means of manufacturing stem cells as they are easier to collect compared to the conventional types of stem cells used in treatment such as cord blood and bone marrow and the yield and proliferation rate can be substantially increased through DTT treatment, addition of the ROCK inhibitor or culture medium exchange.

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